Bergapten attenuates D-galactose-induced immunosenescence in BALB/c mice / 中国药理学与毒理学杂志

OBJECTIVE Bergapten (BG), is a furanocoumarin derived from herbal and citrus extracts can act as antioxidant and selective anticancer agents.The current study aimed to investigate whether bergapten would attenuate immunosenescence and to exploreits immunomodulatory effects on immune responses in D-galactose-induced aging BALB/c mice.METHODS Firstly,mice were given D-galactose(180 mg·kg-1)subcutaneous injections for 30 d.To evaluate the establishment of the aging-related effect in mice, serum samples of BALB/c mice were collected from tail vein. Aging BALB/c mice were freely divided into three groups: negative control group received 1% Tween 80 solution only, named D-gal group. Positive groups were received BG administration at the dose of 20 and 100 mg·kg-1, named D-gal+BG(20)group and D-gal+BG(100)group,respectively.Effects of bergapten on T lympho-cyte proliferation and flow cytometry were assessed by using the splenic cell suspension. Enzyme linked immunospot kits were used to quantitatively determine interferon-γ(IFN-γ)and interleukin-4(IL-4) levels of the isolated serum. Immunophenotype was determined by using mixture of antibodies includ-ing anti-CD3,anti-CD4,and anti-CD8.RESULTS Bergapten(20 mg·kg-1)therapy can modulate immu-nity against viral epidemics and attenuate aging-induced immune deficiency(P<0.01),which was correlat-ed with the decline in the activation of the Th and Tc responses in D-galactose induced aging BALB/c mice.According to the in vivo results,bergapten exposure up-regulated the secretion of IFN-γ and IL-4 in T-helper 1(Th1)and T helper 2(Th2)cells(P<0.05,P<0.01).Additionally,BG(20 mg·kg-1)restored antigen-specific CD4+and CD8+T cells in aging models (P<0.05, P<0.01), which may help to curing chronic infections. CONCLUSION The beneficial effect of bergapten in D-galactose induced aging BALB/c mice may be due to the Th and Tc responses activation.

The design and application of anterior cervical pedicle screw-plate system in lower cervical spine / 中国骨伤

<p><b>OBJECTIVE</b>To explore the applied feasibility of the anterior cervical pedicle screw-plate system in lower cervical spine,in order to provide basic data for clinical application.</p><p><b>METHODS</b>Total thirty-two units (functional spinal unit, FSU) were got randomly from 16 cervical speciments, 8 units in each group of C3,4, C4,5, C5,6 and C6,7. The anterior cervical pedicle screw-plate system was implanted to reconstruct the stability of FSU after discectomy and bone graft. The adaptability was measured between the screw-plate system and vertebral body. X-ray and CT were used to evaluate the accuracy of anterior cervical pedicle screws. The subject will be dissected to identify the situation of involvement if screw perforating the pedicle.</p><p><b>RESULTS</b>Sixty-four anterior pedicle screws were inserted smoothly in the 32 units. The screw and the plate were harmonious locked in the system. The position and length of all screws were satisfactory through X-ray views. However,6 screws perforated the transpedicular (degree 1) according to CT axial views,2 internally cortex and 4 laterally cortex. None perforation was degree 2 or more. None cervical sac compression and nerve root injury was observed in two internal perforation cadavers. One vertebral vein involvement was found in the four lateral perforation screws. The vertebral artery was not pinched though one screw near to the artery.</p><p><b>CONCLUSION</b>The anterior cervical pedicle screw-plate system is adapted to reconstruct in lower cervical spine and it deserved to be used for clinical application.</p>

Recipient dendritic cells modified by RNA interference targeting CD80 and CD86 elicit T cell hyporesponsiveness via enhanced T cell apoptosis / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Despite extensive research, the mechanism of immature dendritic cells (DCs) induced immune hyporesponsiveness remains incompletely understood.</p><p><b>METHODS</b>Recipient DCs from C3H mouse bone marrow cells were incubated with donor antigen from splenic lymphocytes of C57BL/6 mouse; these DCs were transfected with CD80/86 specific siRNA using lentiviral vectors. Flow cytometry was used to evaluate expression of CD80/86 on the antigen-pulsed recipient DCs. Immune regulatory activity was examined by mixed lymphocyte reaction, in which irradiated DCs were cultured with C3H spleen T cells. After the reaction, interleukin (IL)-2, IL-4, IL-10, and interferon (INF)-γ levels of mixed lymphocyte reaction culture supernatant were measured by enzyme-linked immunosorbent assay. The apoptotic T lymphocytes were identified by Annexin V and CD3 staining.</p><p><b>RESULTS</b>There was a significant inhibition of CD80/86 expression in DCs transfected with CD80/86 lentiviral vectors compared with the control groups (P < 0.05), indicating the specificity of RNA interference. Enzyme-linked immunosorbent assay results showed a significant reduction of INF-γ, IL-2 and IL-10 in the CD80/86 lentivirus transfected group compared to the control groups (P < 0.05). There was no significant difference in IL-4 levels between the groups (P > 0.05). We also showed that CD80/86 low DCs loaded with alloantigen (1) stimulated low T cell proliferative responses via the indirect recognition pathway and (2) enhanced apoptotic activity (P < 0.05) in co-cultured T cells.</p><p><b>CONCLUSIONS</b>Lentiviral vector transfection can effectively and specifically knock down target genes in DCs. The CD80/86 low DCs may show tolerogenic activity via induction of T-cell apoptosis, thereby modulating the activity of recipient-derived DCs. The use of this approach may potentially be clinically applicable.</p>

Radiological studies on the best entry point and trajectory of anterior cervical pedicle screw in the lower cervical spine / 中国骨伤

<p><b>OBJECTIVE</b>To explore the best entry point and trajectory of anterior cervical screw in the cervical screw by radiological studies, and provide reference for clincal application.</p><p><b>METHODS</b>From January 2008 to December 2010,50 patients were scanned by cervical CT and confirmed no obvious defect of lower cervical spine. Of them, 27 cases were males and 23 were females, ranged the age from 38 to 83 years ( mean 58.5 years). On horizontal axis, the camber angle of C3-C7 anterior lower cervical pedicle of vertebral arch axis (alpha) and distance between (axial length, AL) of anterior cervical pedicle axial line was measured from C3 to C7. Vertebral were divided into four areas, and from measured side of pedicle of vertebral began to record, orderly 1 to 4, the area of pedicle vertebral arch intersert into vertebral were recorded. On sagittal view, the head or tail angle (beta) and length (sagittal length, SL) of anterior cervical pedicle axial line was also measured from C3 to C7. Vertebral were divided into four areas, and from measured side of pedicle of vertebral began to record, orderly 1 to 4, the area of pedicle vertebral arch arch intersert into vertebral were recorded. The above data were statistically analyzed to find the best entry point and trajectory of anterior cervical screw in the cervical screw and insert pedicle screw.</p><p><b>RESULTS</b>The lateral angle of lower cervical spine was 38 degrees to 45 degrees on transverse plane, C3 to C5 increasing gradually, C5 to C7 decreasing. On sagittal view, C3,C4 pedicle were head tulting, C5 were basic level, C6,C7 were tail. C3 to C5 decreasing gradually, C5 to C7 increasing gradually. C3 to C7 in AL and SL increased gradually. On horizontal axis, the intersection of C3,C4 and C5 were in the second area, the number of C6 in the second and third area were the same, but C7 were in the third area. The intersection in the first and forth area were less. On sagittal view,the intersection of C3,C4 and C5 were in the first area,the number of C6 in third and forth area were less. Six pedicle screws of 3 cases were insert into lower cervical spine, and obtained good effects, no complications occurred.</p><p><b>CONCLUSION</b>The best entry point of C3,C4 and C5 were located in the center line and slightly to opposite vertebral body side and upper 1/4 area; C7 were located the vertebral body side and upper 2/4 area; C6 were located between them. The best insertion point were extraversion 38 degrees to 45 degrees, C3 to C5 increased graduallly, C5 to C7 decreased on horizontal axis; On sagittal view, C3,C4 for head 5 degrees to 10 degrees, C5 were basic level, C6,C7 for tail 5 degrees to 10 degrees. The anterior cervical pedicle screw for lower cervial spine is a good and feasible internal fixation.</p>

Preliminary clinical study of anterior pedicle screw fixation for lower cervical spine injuries / 中华创伤杂志

Objective To investigate the preliminary clinical application of anterior pedicle screws in treatment of the lower cervical spine injuries. Methods The study involved 22 patients with lower cervical spine injuries treated with anterior pedicle screw technique from January 2009 to December 2011.X-ray and CT images of the patients were taken postoperatively to evaluate whether the position,orientation and length of the screws were appropriate and whether there involved pedicle cortex perforation,transverse foramen or spinal canal invasion.MRI was also performed to ascertain the situation of decompression,the formation of epidural hematoma and the degeneration of cervical spinal cord.The improvement of JOA score was compared statistically. Results All the patients were followed up for 3-36 months ( mean,15.5 months).A total of 44 anterior lower cervical screws were implanted and all screws were inserted smoothly.All the patients had bone healing after average 4.5 months,which showed no loosening,prolapse or breakage of the screws or no loosening of the plate.One patient showed hoarseness postoperatively,which was probably due to the long-term distraction of recurrent laryngeal nerve.The symptom disappeared at around three weeks after neurotrophic support.Two patients complained of dysphagia postoperatively,and were informed of keeping a soft diet.The symptom was remarkably alleviated after three months or so.The X-ray films indicated satisfactory position of the implanted screws.Before discharge,the transaxial CT images showed that two screws perforated the medial pedicle cortex ( 1 ° ) and that two screws perforated the lateral cortex (1 °) and affected the medial margin of the transverse foramen.Before discharge,the MRI indicated obvious alleviation of the anterior spinal cord compression in all patients and a small amount of anterior epidural hematoma in four patients,with no obvious spinal cord compression.At 12 months after operation,the MRI showed significant improvement of spinal cord degeneration in three patients.JOA score was increased from preoperative (8.5 ± 0.7) to ( 14.5 ± 0.8 ) before discharge (P ＜0.01 ).JOA score was (15.7 ±0.7) at six months postoperatively,which was significantly improved as compared with that before discharge. Conclusions Anterior pedicle screw fixation is a reliable and safe method for reconstruction of the anterior lower cervical injuries.Surgical indications should be strictly controlled in its clinical application.

A analysis on the effect of with or without patellar replacement in total knee arthroplasty / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the outcome of total knee arthroplasty with or without resurfacing of the patella with particular attention to knee score, knee function score and incidence of postoperative anterior knee pain, providing the basis for the choice of surgical procedure.</p><p><b>METHODS</b>CNKI, PubMed, ScienceDirect, Highwire and other databases were searched for the randomized controlled trials relevant to the patellar with or without replacement in total knee replacement arthroplasty between 1998 and 2010, evaluating of the methodological quality of included studies and extracting valid data.</p><p><b>RESULTS</b>The 80 citations were identified as related to patellar resurfacing during total knee arthroplasty, 13 articles meet all inclusion criteria for this study. The incidence of postoperative anterior knee pain is greater in knees without replaced patellas (RR = 0.78, 95%CI: 0.61 - 0.99, P = 0.04). No differences are observed between the 2 groups for knee score and knee function score. Knee score (WMD = -0.49, 95%CI: -1.79 - 0.81, P = 0.46), knee function score (WMD = 1.10, 95%CI: -1.77 - 3.98, P = 0.45).</p><p><b>CONCLUSIONS</b>The patella replacement can significantly reduce the incidence of postoperative anterior knee pain. There is no difference in the knee score and knee function score between two groups.</p>

Comparison of three techniques of the transarticular screw placement in the lower cervical spine / 中国骨伤

<p><b>OBJECTIVE</b>To compare three techniques of the transarticular screw placement in the lower cervical spine.</p><p><b>METHODS</b>The techniques of transarticular screw placements used by Takayasu (group A), Dalcanto (group B) and Klekamp (group C) were applied in twenty-four cervical specimens, among which 14 specimens were male and 10 specimens were female, ranging in age from 55 to 80 years, with an average of 68 years. The entry point of Takayasu technique was between the superior and median third of the vertical medial line of the lateral mass with 60 to 80 degrees caudal tilt and 0 degrees lateral tilt. Dalcanto technique started 2 mm caudal to the midpoint of lateral mass with 40 degrees caudal tilt and 20 degrees lateral tilt. Klekamp technique inserted the screws with the starting point being 1 mm medial and 1 mm inferior to the midpoint of the lateral mass with 40 degrees caudal tilt and 20 degrees lateral tilt. The splits of facets, the encroachment of the cervical nerve roots and vertebral arteries, and no-involving facets screws were observed and analyzed.</p><p><b>RESULTS</b>One hundred and ninety-two transarticular screws were implanted on both sides in twenty-four cervical cadavers, sixty-four for each group. There were twenty-five splits of inferior facets in group B, 2 splits in group C and none in group A. No superior facets were found to be broken in all cases. The difference of splits between group B and the other two groups was significant (Chi2AB = 31.07, P < .001; Chi2BC = 24.83, P < 0.01), while there was no statistical difference between group A and C (Chi2AC = 2.03, P > 0.05). Thirty-six screws encroached the vertebral arteries in group A, none in group B and C. There was significant statistical difference between group A and the other two groups (Chi2AB = 50.09, P < 0.01; Chi2AC = 50.09, P < 0.01). Forty anterior branches of lower cervical nerve roots were involved in group A, five in group B and three in group C. There were statistical differences between group A and the other two groups (Chi2AB = 41.98, P < 0.01; Chi2AC = 47-94, P < 0.01), and there was no statistical difference between group B and C. Sixteen posterior branches of lower cervical nerve roots were encroached in group A, 18 in group B and 14 in group C. There was statistical difference among the three groups (Chi2AB = 0.16, P > 0.05; Chi2AC = 0.17, 0.05; Chi2 = BC = 0.67, P >0.0 ). Although all screws went through facets in the study except for 5 in group A, there were no statistical differences between each groups Chi2AB k =3.33, P >0.05; X2A C =3.33, P> 0.05).</p><p><b>CONCLUSION</b>There are high risk of injury of anterior branch of cervical nerve root and vertebral artery if the screws are too long and the Takayasu's technique is used. However, the rate of facet split is high if the Dalcanto's technique is applied. Klekamp's technique is value to be applied.</p>

Safety factors for anterior pedicle screw fixation tunnel in axis / 中华创伤杂志

Objective To investigate feasibility and safety of anterior pedicle screw fixation tunnel in the axis so as to provide theoretic evidence for further clinical application.Methods Thirty-two dry axis specimens were used for anterior pedicle screw placement,and the length of pedicle crews tunnel was measured by a digital caliper.The extraversion angle and the downslope angle were determined by CT cross-sectional scanning and reconstruction.Results All the anterior pedicle screws were positioned well,with no perforation of the pedicle.The anchoring point was at the vertex between the vertebral body and the superior articular process,with axis pedicle length of(28.4 ± 2.5)mm,the extraversion angle of (28.5 ±2.3)°and the downslope angle of(15.5 ±2.0)°.Conclusions Anterior pedicle screw fixation tunnel is feasible in the axis and can keep away from some important anatomy structures including transverse foramen,vertebral artery groove and vertebral canal to provide large safety space.

Study on the spiral CT reconstruction in the diagnosis and treatment of pelvic ring fractures / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the clinical value of spiral CT with multi-planar reconstruction (MPR) and three diamensions reconstruction (3D) in the diagnosis and treatment of pelvic ring fractures.</p><p><b>METHODS</b>Fifty-seven patients with pelvic ring fractures were examined with digital radiography and spiral CT in our hospital from April 2004 to April 2009. According to Tile classification, there were thirty-eight cases in type A, twelve in type B and seven in type C. Expectant treatment was used in type A, while surgery, open reduction internal fixation or percutaneous lag screw internal fixation technique for sacroiliac joint due to the displacement of fractures, for type B and C. Three days to twenty-seven months after operation, spiral CT examinations were used to evaluate the location of internal fixation. Cross-check analysis of images of digital radiography and spiral CT was performed before and after operation.</p><p><b>RESULTS</b>Five posterior and three anterior pelvic ring fractures were diagnosed as suspected fractures. Nine posterior and three anterior pelvic ring fractures were missdiagnosed according to plain radiographs, which were corrected by spiral CT examination. According to the postoperative imageology evaluation, the results were excellent in 15, good in 3 and bad in 1. According to clinical evaluation, 16 cases were excellent, 3 good.</p><p><b>CONCLUSION</b>Spiral CT with multi-planar reconstruction (MPR) and three diamensions reconstruction (3D) has clinical values for precise diagnosis and treatment for the complex pelvic ring fractures.</p>

Clinical application of multi-spiral CT thinner scanning and reconstruction in the diagnosis of atlantoaxial fracture and dislocation / 中国骨伤

<p><b>OBJECTIVE</b>To evaluate the value of multi-spiral CT thinner scanning and three-dimensional and multi-planar reconstruction in the diagnosis of atlantoaxial fracture and dislocation.</p><p><b>METHODS</b>Sixty-nine patients suffered from atlantoaxial injuries included 54 males and 15 females with an average age of 46.5 years ranging from 17 to 73. Roentgenograph, multi-spiral CT thinner scanning, three dimensional reconstruction (3D) and multi-planar reconstruction (MPR) were applied for patients before and after surgery.</p><p><b>RESULTS</b>Multi-spiral CT thinner scanning and reconstruction provided clear images for all subjects. There were 49 odontoid bone fractures (Anderson type II 35, type III 14), 9 cases combined injuries of C1 and C2 (Jeffson's fracture associated with Hangman fracture), 6 of C1 burst fractures and 5 of C2 vertebral body fractures. While only 38 cases (38/69) were correctly diagnosed according to roentgenographs, 23 missed diagnosis, 6 couldn't be diagnosed clearly and 2 misdiagnosis. The position of internal fixation could be seen clearly in CT imaging after surgery. There were two screws perforated the lateral wall of C1 and three perforated the superior wall.</p><p><b>CONCLUSION</b>The multi-spiral CT thinner-scanning, three dimensional reconstruction (3D) and multi-planar reconstruction (MPR) provide clear imagings to diagnose the details of atlantoaxial fractures and dislocations, and to select the therapeutic regimen. It can also be used to check the position of the internal fixation after surgeries. So it can be the first eikonic choice for atlantoaxial injuries.</p>

Imageology analysis of perilunar dislocation and its clinical application / 中国骨伤

<p><b>OBJECTIVE</b>To analyze imageology manifestation of perilunar dislocation, to improve recognition and diagnose of the perilunar dislocation.</p><p><b>METHODS</b>Fifty-six patients suffered from perilunar dislocation injuries were selected in our group, fifty-five male and one female, ranging in age from 18 to 47 years, with an average of 32.4 years. Roentgenograph, thin slice multi-spiral CT scanning, three dimensional reconstruction (3D) and multi-planar reconstruction (MPR) were underwent for patients before and after surgery.</p><p><b>RESULTS</b>Thin slice multi-spiral CT thinner scanning and reconstruction provided clear images for all subjects, in which 56 patients had dorsal perilunate dislocations, 11 patients had transscaphoid perilunate dislocations, 10 patients had perilunar dislocation without carpus fractures, 26 patients had perilunar dislocation through centrale and pyramidal bone (one combined with avulsion fracture of pisiform bone), 6 patients had trans triangular bone perilunar dislocation, 3 patients had trans capitate bone perilunar dislocation, 24 patients had perilunar dislocation combined with distal fractures of radial and ulnar, 4 patients had perilunar dislocation combined with dislocation of wrist joint.</p><p><b>CONCLUSION</b>To be familiar with normal imageology anatomy of wrist joint and to master imageology characteristic of all types of perilunar dislocation are the basis of final diagnosis and prompt treatment. The thin slice multi-spiral CT scanning, three dimensional reconstruction (3D) and multi-planar reconstruction (MPR) provide clear images to diagnose the details of perilunar dislocation, and to select the therapeutic regimen. So it can be the first eikonic choice for perilunar dislocation.</p>

Study of Rhubarb anti-Yersina pestis based on DNA microarray / 中国地方病学杂志

Objective To establish a method for studying molecular mechanism of Rhubarb inhibiting anti-Yersinia pesti based on DNA microarray.Methods A whole genome DN A microarray containing 4005 annotated genes of Yersiniapesti Was used.The minimal inhibitory concentration(MIC)of Rhubarb to Yersiniapestiwas determined by liquid dilution method.The gene expression profile of Yersinia pesti was performed after the exposure to Rhubarb at a concentration of 10×MIC for 30 minutes.The total RNA extracted and purified from Yersinia pesti Was reversely transfected to cDNA and labeled by Cy3-Cy5 dye.The labeled probes were hybridized to the microarray anti the results were obtained by a laser scanner and the microarray data was confirmed by real-time quantitative RT-PCR.Results The platform of the DNA microarray-based bacteria transcriptional profile was established.A total of 498 genes of Yersinia pesti changed significantly in response to Rhubarb.Among them.358 genes were up-regulated,140 down-reguated.Conclusions The whole genome DNA microarray can be used in the studying of molecular anti-Yersinia pesti mechanism of Rhubarb.

Global gene expression of berberine against Yersiniapestis in vitro / 中国地方病学杂志

Objective To investigate the antibacterial molecular mechanism of Traditional Chinese Medicine Coptis rhizome against Yersinia pestis(Y.pestis).Methods The method based on whole genome DNA micrnarray of Y.pestis was used.The minimal inhibition concentration(MIC)of berberine to Y.pestis was determined with liquid dilution method.Then gene expression profile of Y.pestis was performed after exposed to berberine at the concentration of 10×MIC for 30 minutes.Total RNA extracted and purified from Y.pestis and reverse-transcribed to cDNA,then labeled by Cy-dye.Finally,the labeled probes were hybridized to the microarray and the results were obtained by a laser scanner and analyzed by the SAM software.Results The gene expression profile data revealed that the response of Y.pestis to berberine was a global phenomenon.A total of 360 genes changed significantly.Among them,333 genes were up-regulated,27 down-regulated.These differentially expressed genes were further classified into 24 different functional categories based on the genomie annotation of Y.pestis CO92,in which the number of mainly related genes were 83,75 and 48,including cell envelop,unkown,transport/binding proteins functions.The 40 genes related to the metabolism were upregulated,which was a remarkable change.Conclusion Our results have revealed the general gene expression changes of Y.pestis in response to berberine and demonstrated the antibacterial molecular mechanism of the Coptis rhizome.The major mechanism of Y.pestis in response to berberine is the upregulation of genes related to the metabolism.

Effect of Metalloproteinases Inhibitor on Expression of MMP-3 in Degenerated Lumbar Intervertebral Disc / 中国康复理论与实践

@# ObjectiveTo explore the effect of metalloproteinases (MMPs) inhibitor on expression of MMP-3 in degenerated lumbar intervertebral disc.MethodsThe animal model of degenerated lumbar intervertebral disc was established with rats. The 32 rats were randomly divided into the experimental group and control group with 16 animals in each group. From the fourth week after operation, the animals of the experimental group were injected with tetracycline 25 mg per day, those of the control group with saline. Two weeks late, the tissue of degenerated lumbar intervertebral disc was taken and the expression of MMP-3 was tested by immumohistochemistry and Western-bloting.ResultsThe expression of MMP-3 in the experimental group decreased and significantly different from the control group ( P<0.05).ConclusionMMPs inhibitor can decrease expression of MMP-3 in degenerated lumbar intervertebral disc.

Significance and role of increased expression of extracellular matrix metalloproteinase induced in the aseptic loosening of prostheses / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the expression of extracellular matrix metalloproteinase induced (EMMPRIN) in the interface tissue, and explore the role of EMMPRIN in the aseptic loosening of prostheses.</p><p><b>METHODS</b>Immunohistochemistry was performed to characterize the EMMPRIN-expressing cells at sites of interface tissue around aseptic loosened hip prostheses in 16 cases. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to study the existence of EMMPRIN mRNA in interface tissue samples. And it was followed up by computer assisted image analysis in order to detect the A values of their expression. Synovium of hip joint of 8 femoral neck fracture were in control group.</p><p><b>RESULTS</b>Strong immunostaining of EMMPRIN was found in the macrophages and fibroblasts of lining-like layers and vascular endothelium of synovial membrane-like interface tissue around loosened prostheses. Expression of EMMPRIN was significantly higher in interface tissue than the control synovium (z=-3.252, P=0.001). RT-PCR of interface tissue samples disclosed the presence of EMMPRIN mRNA of 14 cases. In interface tissue, the A value of EMMPRIN increased significantly compared to control synovium (P<0.01).</p><p><b>CONCLUSION</b>Over-expression of EMMPRIN up-regulates the production of matrix metalloproteinase (MMPs) in the interface tissue. And it can promote the bone destruction around prostheses. Thereby it may be one of methods to prevent and treat aseptic loosening of prostheses by repression the biology activity of EMMPRIN.</p>

The clinical study on application of using a novel blockade technique for gastric cancer to decrease blood-borne metastasis of cancer cells / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the effect of a novel blockade technique for gastric cancer on blood-borne metastasis of gastric cancer cells to portal vein.</p><p><b>METHODS</b>Twenty-three cases of gastric cancer were divided into routine operation group (8 cases intraoperatively without blockade technique) and blockade group (15 cases with blockade technique). Blood samples from portal vein pre- and intraoperatively, as well as gastroepiploic vein limited within the blockade area were obtained to detect CK19 mRNA expression by using RT-PCR technique.</p><p><b>RESULTS</b>Before the dissection of gastric lesion, the overall positive rate of CK19 mRNA expression in portal vein blood is 34.7% (9/23), including 37.5% (3/8) in routine operation group and 33.3% (5/15) in blockade group. While the course of tumor resection, those positive rates were 87.5% (7/8) in routine operation group and 6.7% (1/15) in blockade group respectively (P < 0.05). CK19 mRNA expression in the right gastroepiploic venous blood limited within the blocking area was all positive in 15 cases of blockade group.</p><p><b>CONCLUSION</b>This blockade technique can be used effectively to block the intraoperative spread of gastric cancer cells, thus prevent blood-borne metastasis due to operative manipulation.</p>

Study on purification of total flavonoids from seed residue of Hippophae rhamnoides with macroporous resin / 中国中药杂志

<p><b>OBJECTIVE</b>To study the conditions and parameters of purifying total flavonoids from seed residue of H. rhamnoides.</p><p><b>METHOD</b>Absorption capacity of three resins for total flavonoids was compared. With the yield and purity as indexes, the process of absorbing and purifying total flavonoids from seed residue with D101 macroporous resin absorbent was selected by orthogonal design.</p><p><b>RESULT</b>The D101 resin was the best of the three resins and its absorption capacity was determined to be 63.00 mg x g(-1) wet resin. The optimum process condition was 30% ethanol as eluting solvent, wet resin vs crude herbs: 2:1, diameter vs height: 1:10, eluting solvent vs crude herbs: 10:1, absorbing time for 3 h. The yield of total flavonoids from seeds residue of H. rhamnoides by this process was 2.39%, and the purity reached 64.81%.</p><p><b>CONCLUSION</b>The process is simple and convenient and the regeneration of resin is easy. So this method of purification is advisable.</p>